Situation: You need some text boxes disable when gridview is in edit mode.
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Solution:
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1) Bind the Data without Sqldatasource Control
2) In Row Editing write the code:

protected void GridView1_RowEditing(object sender, GridViewEditEventArgs e)
{
GridView1.EditIndex = e.NewEditIndex;
gridbind();
TextBox tx_chdets = (TextBox)GridView1.Rows[e.NewEditIndex].FindControl(“TextBox1”);
tx_chdets.Readonly=true;

}

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